METHOD OF DETERMINING DIBUNOL IN BIOLOGICAL SPECIES Russian patent published in 2000 - IPC

Abstract RU 2157992 C1

FIELD: forensic medicine. SUBSTANCE: method is designed for use when determining dibunol in tissues and body fluids as well as when investigating farmacodynamics in various medicinal forms. One determines time of extraction of dibunol from biological objects performing extraction with isopropyl alcohol under reflux followed by thin-layer chromatography involving 9:1 chloroform/acetone solvent system and development in iodine vapors. After elution with ethanol, one measures spectrophotometrically weight concentration of dibunol at wavelength 276 nm using extinction coefficient value 101.5 (for 1 cm cuvette). EFFECT: increased determination accuracy and simplified procedure. 6 ex

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RU 2 157 992 C1

Authors

Likhoded V.A.

Nasyrov Kh.M.

Shikova Ju.V.

Zyrjanov S.K.

Chernov N.V.

Shikov A.N.

Dates

2000-10-20—Published

1999-02-22—Filed