REAGENT FOR ADENOSINE 5'-TRIPHOSPHATE ASSAY Russian patent published in 2001 - IPC

FIELD: analytical biochemistry. SUBSTANCE: reagent for determination of adenosine 5'-triphosphate has luciferase isolated from E. coli recombinant cells containing plasmid with gene encoding firefly luciferase. Luciferase is purified by precipitation with 35-75% ammonium sulfate and immobilized on polysaccharide carrier. Reagent has also luciferin, magnesium sulfate, mixture of tris-(hydroxymethyl)-aminomethane and acetic acid as a buffer mixture, mixture of ethylenediaminetetraacetate sodium with dithiothreitol as a stabilizing agent and bovine serum albumin, trehalose and water as an additional stabilizing agent. In adenosine 5'-triphosphate assay reagent provides increase of sensitivity (minimal detectable concentration of ATP is 10^-14 M) and significant decrease of background signal intensity. Reagent shows stability at storage both in an aqueous suspension and in lyophilized state. EFFECT: improved method of assay, increased sensitivity. 2 cl, 3 tbl, 5 ex