FIELD: biochemistry.
SUBSTANCE: invention relates to the field of biochemistry. Disclosed is a complex of reagents for the adenosine-5'-triphosphate (ATP) quantitative analysis. Complex includes luciferin and an enzyme preparation. Enzyme preparation contains firefly luciferase, a buffer solution, dithiothriotol and bovine serum albumin stabilizing additives. At that, the dose of each of the reagents for one quantitative analysis is immobilized separately. Thus, the enzyme preparation is immobilized in the gelatin gel with a quantitative ratio of firefly luciferase and gelatin from 1:50 to 1:90, and luciferin is immobilized in a gelatin gel in a quantitative ratio of 1:15 to 1:50 or in a starch gel at a quantitative ratio of 1:40 to 1:150.
EFFECT: invention provides an increase in the stability of the reagents for determining ATP during storage and use, as well as simplifying the reagent preparing procedure.
1 cl, 3 dwg, 3 ex
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