FIELD: medicine, cardiology, therapy, biology, biochemistry. SUBSTANCE: invention relates to development of incubation medium used for assay of catalase activity in plasma blood. Medium has containing phosphate buffer K2HPO4/NaH2PO4, 60 mM/l; pH = 7; ammonium chloride, 500 mM/l; Triton X-100, 0.769 mM/l; H2O2, 11.6 mM/l. Indicated composition of incubation medium provides to determine kinetics of catalase activity in plasma blood by photometric method at λ = 405 nm, t = 37 C for 9 min with high precision. EFFECT: enhanced quality of incubation medium, improved precision of assay. 1 cl, 1 ex
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Authors
Dates
2001-10-27—Published
1999-11-15—Filed