FIELD: biotechnology, biochemistry. SUBSTANCE: isolation of immunoglobulins of class G is carried out by 5-fold reprecipitation with polyethylene glycol-6000. Animals are immunized with isolated immunoglobulins of class G for preparing antispecies serum. Specific antibodies are isolated from antispecies serum and their activity is determined by titration in immunoenzyme analysis with homologous antigens. Method allows to enhance activity and specificity of the end product. EFFECT: improved preparing method. 3 tbl, 3 ex
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Authors
Dates
2003-04-20—Published
2000-12-04—Filed