FIELD: veterinary virology, biotechnology. SUBSTANCE: the present innovation deals with preparing antigenous material as 5-10%-virus-containing suspension out of the liver of 5- 50-d-aged chickens or primary hepatic cell culture of SPF-hens' embryos infected with KR95 strain N 114-DEP of hens' hydropericarditis syndrome virus (VGNKI collection) at biological activity of 3.25-5.0 lg TЦД50/cm3. Virus is cultivated in chicken's body or cell culture for 48-120 h. To prepare vaccine one should apply virus at biological activity of 4.25-5.0 lg TЦД50/cm3. After purifying virus-containing suspension against ballast admixtures due to known technique, it should be supplemented with ethylenimine dimer at concentration of 0.15-0.4% for viral inactivation. Purified and avirulent antigenous material is combined with oily adjuvant in efficient ratio. The vaccine in question is of higher antigenous and immunogenous activity and safe. EFFECT: higher efficiency. 14 cl, 7 ex, 6 tbl
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Authors
Dates
2003-10-10—Published
2002-01-22—Filed