FIELD: chemistry of proteins, medicine, oncology, pharmacy, medicinal industry. SUBSTANCE: invention concerns to polypeptides showing specificity for CD19 and CD3 and their application. Invention involves a single-chain multifunctional polypeptide with specificity for antigens CD19 and CD3 of the formula: VICD19-VHCD19-VHCD3-VL-CD3, polynucleotide encoding this polypeptide, expressing vector, the strain of culture cells, method for preparing polypeptide. Invention relates to pharmaceutical composition used for treatment of malignant diseases of B-cells, diagnostic composition, method for detection of activators and inhibitors of T-cell activation or stimulation. Invention relates to method for preparing a pharmaceutical composition designated for treatment of malignant diseases of B-cells and to method for treatment of malignant diseases of B-cells or depletion of B-cells and to method for retardation in development of pathological state. The advantage of invention involves the development of methods useful for treatment of B- cells-mediated diseases. EFFECT: valuable medicinal properties of polypeptides. 18 cl, 22 dwg, 2 tbl, 7 ex
| Title | Year | Author | Number |
|---|---|---|---|
| BISPECIFIC ASYMMETRIC HETERODIMERS COMPRISING ANTI-CD3 CONSTRUCTS | 2013 |
|
RU2650868C2 |
| COMPLEMENTARITY-DETERMINING REGIONS FOR BINDING CD3 AND A BISPECIFIC ANTIGEN-BINDING MOLECULE CONTAINING THEREOF | 2019 |
|
RU2738802C1 |
| TRIFUNCTIONAL ANTIGEN-BINDING MOLECULE | 2015 |
|
RU2753882C2 |
| CYTOTOXICITY-INDUCING THERAPEUTIC AGENT | 2011 |
|
RU2774414C2 |
| CYTOTOXICITY-INDUCING THERAPEUTIC AGENT | 2011 |
|
RU2663123C2 |
| MULTIVALENT ANTIGEN-BINDING FV MOLECULE | 2011 |
|
RU2613368C2 |
| ANTIBODY AND CHEMOKINE CONSTRUCTS AND USES THEREOF IN IMMUNOLOGICAL DISORDERS | 2001 |
|
RU2252786C2 |
| BIOSPECIFIC ANTIGEN-BINDING MOLECULES | 2012 |
|
RU2650775C2 |
| BISPECIFIC ANTIBODIES AGAINST CD3*CD19 | 2015 |
|
RU2651776C2 |
| PSMA×CD3 BISPECIFIC SINGLE-CHAIN ANTIBODY HAVING INTERSPECIES SPECIFICITY | 2009 |
|
RU2559531C2 |
