MULTIVALENT ANTIGEN-BINDING FV MOLECULE Russian patent published in 2017 - IPC C07K16/46 

FIELD: biochemistry.

SUBSTANCE: present invention relates to biochemistry and immunology, in particular, to a dimeric antigen-binding molecule, which is crosslinked with antigen A and antigen B. Said molecule consists of two polypeptide chains, each of which comprises a variable light chain domain, specific to antigen A (V_LA), variable heavy chain domain, specific to antigen B (V_HB), variable light chain domain, specific to antigen B (V_LB) and a variable heavy chain domain, specific to antigen A (V_HA). Domains are arranged in following order V_LA-V_HB-V_LB-V_HA. V_LA of first polypeptide chain is linked to V_HA of second polypeptide chain to form an antigen-binding site for antigen A, V_HB of first polypeptide chain is bonded to V_LB of second polypeptide chain to form an antigen-binding site for antigen B, V_LB of first polypeptide chain is linked to V_HB of second polypeptide chain to form an antigen-binding site for antigen B and V_HA of first polypeptide chain is linked to V_LA of second polypeptide chain to form an antigen-binding site for antigen A. Present invention discloses a composition for treating diseases, in which antigens A and B are expressed and containing said antigen-binding molecule.

EFFECT: present invention due to such arrangement of variable domains in antigen-binding molecule V_LA-V_HB-V_LB-V_HA from N-terminal to C-terminal of polypeptide, makes it possible to crosslink between antigens A and B, improves efficiency of binding with target antigens.

15 cl, 7 dwg, 1 tbl, 2 ex