METHOD FOR PREPARING ENDONUCLEASE RESTRICTION SST 12I Russian patent published in 2004 - IPC

Abstract RU 2233877 C2

FIELD: biochemistry, microbiology, genetic engineering.

SUBSTANCE: invention relates to isolation and purification of site-specific endonuclease restriction synthesized by microorganisms of genus Streptomyces, namely, to preparing site-specific endonuclease restriction Sst 12I that recognizes and cleaves the nucleotide sequence: 5'-CTGCAG-3'. Method involves disruption of cells by ultrasonic oscillation, fractional thermal treatment (3 times for 3 min) at temperature 60 C, not above, centrifugation, chromatography on phosphocellulose P-11 column and concentrating against 50% glycerol solution. The yield of enzyme is 500000 U/10 g of biomass. This method provides preparing enzyme without impurities of nonspecific nucleases and phosphatases.

EFFECT: improved preparing method.

3 dwg, 3 ex

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RU 2 233 877 C2

Authors

Ushakova T.A.

Puchkova L.I.

Polushkina A.F.

Kuvshinov V.N.

Repin V.E.

Dates

2004-08-10—Published

2001-11-12—Filed