FIELD: biotechnology.
SUBSTANCE: invention is a recombinant strain of bacteria Escherichia coli N41 (pBpuN4/MR), which is obtained by transformation of the strain Escherichia coli ER2267 of plasmid pBpuN4/MR N41, obtained based on plasmid pUC19 and containing the gene encoding DNA-methyltransferase M.BpuN4I, methylating one of cytosines in position C5 in the sequence 5'-GGNNCC-3', and the restriction endonuclease gene BpuN4I. This recombinant strain of bacteria Escherichia coli N41 (pBpuN4/MR) is the producer of the site-specific endonuclease of restriction BpuN4I which recognises DNA sequence 5'-G^GNNCC-3'/3'-CCNNG^G-5' and cleaves both its chains after the first guanine to form 5'-protruding four-nucleotide cohesive ends.
EFFECT: invention enables to obtain restriction endonuclease of the predetermined specificity with high yield.
3 dwg, 4 ex
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