METHOD OF RESTRICTION ENDONUCLEASE PRODUCING Russian patent published in 1999 - IPC

Abstract RU 2136749 C1

FIELD: biotechnology, biochemistry, microbiology. SUBSTANCE: method involves cells disruption by ultrasonic oscillation, thermal treatment at 58-60 C for 5-10 min, not less, centrifugation, chromatography on DEAE-cellulose (DE-52), an additional purification on phosphocellulose P-11, heparin-Sepharose and the concentration against 50% glycerol solution. The yield of enzyme is 170 000-200 000 U/10 g of wet biomass. Method ensures to obtain the enzyme no containing impurities of nonspecific nucleases and phosphatases and at the low level of protein. EFFECT: improved method of producing. 4 cl, 1 tbl, 2 dwg, 4 ex

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RU 2 136 749 C1

Authors

Puchkova L.I.

Mikhajlova V.K.

Dates

1999-09-10—Published

1997-10-06—Filed