FIELD: biotechnology, medicine.
SUBSTANCE: invention relates to media used for cryoconservation of human and animal cells. Medium contains calf embryo serum, dimethylsulfoxide, medium RPMI-1640, antibiotics, acetamide, D-glucuronic acid, lecithin, cholesterol, linoleic acid taken in the definite ratio of components. Invention provides enhancing viability of cells due to enhancement of their resistance to cryoconservation and reducing toxic effect of cryoprotecting agent.
EFFECT: valuable properties of medium.
2 tbl, 4 ex
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Authors
Dates
2004-10-10—Published
2002-05-06—Filed