FIELD: biotechnology, medicine, transplantation methods.
SUBSTANCE: claimed medium contains medium RPMI-1629 9 ml, 10 % polyvinyl pyrrolidone 1 ml, calf fetal serum N K055 5 ml, potassium orotate 0.75 g, 40 % glucose solution 1 ml, pyruvate 0.001 muM in 1 ml 0.1 ml, linoleic acid 0.016 muM in 1 ml 0.4 ml, pH 7.4. Medium of present invention provides high viability (88.2 ± 1.2 %) cells of Langerhans islets during storage in frozen state for 6 months.
EFFECT: simplified method for cell preparation; decreased cell traumatizing.
1 ex
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