FIELD: biotechnology, chemistry of proteins, protein engineering.
SUBSTANCE: invention relates to expressing vector that can be used in one-step procedure for purification. The recombinant protein is produced as a fused protein with lectin, being especially with discoidin, as a marker in purification procedure. Fused protein can be purified on chromatography column with Sepharose 4B using galactose as an eluent. Invention provides alleviating and accelerating purification of polypeptide and to reduce cost by using cheap resins.
EFFECT: improved producing method.
15 cl, 6 dwg
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Year |
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Number |
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2008 |
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|
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2012 |
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