FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology. DNA construct is disclosed, which codes fused precursor protein, in which auxiliary amino acid sequence is bound to N-end of mature target polypeptide sequence with transition site intended for identification and splitting of hybrid precursor by specific protease to form non-modified protein of interest. Above site represents truncated at N-end by 23 amino acid residues SUMO protein of S. cerevisiae (Smt3), which includes site of splitting with SUMO-hydrolase (Ulp1). On basis of DNA construct coding version of precursor, which comprises glutathione-S-transferase (GST) as auxiliary sequence and negatively charged peptide and human interferon (IFN) α2b as target protein recombinant E. coli strain is obtained. Method of producing non-modified IFN α2b using above E. coli strain is also disclosed.
EFFECT: group of inventions enables to obtain target protein with high purity and activity.
7 cl, 9 dwg, 2 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| HYBRID PROTEIN (VERSIONS), BACTERIAL STRAIN ESCHERICHIA COLI - HYBRID PROTEIN PRODUCER (VERSIONS) AND METHOD FOR PRODUCING METHIONINE-FREE HUMAN INTERFERON ALPHA-2 | 2011 |
|
RU2453604C1 |
| FUSION PROTEIN, ESCHERICHIA COLI STRAIN BEING FUSION PROTEIN PRODUCER AND METHOD FOR PRODUCING METHIONINE-FREE HUMAN INTERFERON ALPHA-2b OF SUCH FUSION PROTEIN | 2010 |
|
RU2441072C1 |
| METHOD FOR PREPARING PROTEINASE Ulp275 | 2011 |
|
RU2451076C1 |
| PLASMID VECTOR PRH15A FOR PRODUCING METHIONINE-FREE INTERFERON ALPHA-2B, BACTERIAL STRAIN ESCHERICHIA COLI BL21 DE3 - PRODUCER OF METHIONINE-FREE INTERFERON ALPHA-2B AND METHOD FOR PRODUCING METHIONINE-FREE INTERFERON ALPHA-2B | 2017 |
|
RU2697375C2 |
| METHOD FOR PRODUCTION OF TYPE 2 PIG CIRCOVIRUS CAPSID PROTEIN AND PHARMACEUTICAL COMPOSITION CONTAINING IT | 2015 |
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RU2701816C1 |
| RECOMBINANT PLASMID DNA PSMT3_HCRG21 ENCODING A HYBRID PROTEIN SMT3-HCRG21, BACTERIAL STRAIN ESCHERICHIA COLI BL21(DE3)/PSMT3_HCRG21 - PRODUCER OF HCRG21 ANALGESIC PEPTIDE AND METHOD OF PRODUCING RECOMBINANT HCRG21 ANALGESIC PEPTIDE | 2022 |
|
RU2798545C1 |
| TEMPERATURICALLY INTELLIGENT MUTANT INTEIN FOR THE INSOLUBLE EXPRESSION OF THE PRECURSOR OF THE TARGET PROTEIN | 2015 |
|
RU2619217C1 |
| METHOD OF OBTAINING BIOLOGICALLY ACTIVE RECOMBINANT PROTEINS | 2022 |
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RU2807615C2 |
| METHOD OF PRODUCING RECOMBINANT SPIDER-WEB PROTEIN, FUSED PROTEIN, RECOMBINANT DNA, EXPRESSION VECTOR, HOST CELL AND PRODUCER STRAIN | 2010 |
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RU2451023C1 |
| STRAIN OF BACTERIA Escherichia coli JM109/pHINS21 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN AND METHOD FOR PRODUCTION OF HUMAN PROINSULIN | 2007 |
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RU2376368C2 |
