FIELD: biotechnology, biochemistry, molecular biology.
SUBSTANCE: raffinose synthase is prepared by culturing the host-cell transformed with vector comprising raffinose synthase gene. Prepared raffinose synthase is isolated from cultural liquid. Partial nucleotide sequence of gene encoding raffinose synthase is used as a probe or primer. Invention allows carrying out the modulation of level of expression and activity in plants.
EFFECT: improved preparing method, valuable properties of gene and enzyme.
24 cl, 8 ex
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Authors
Dates
2004-12-27—Published
1999-04-29—Filed