FIELD: medicine, laboratory diagnostics.
SUBSTANCE: the suggested method is based upon registration the rate of hemolysis in isotonic water solution that contains erythrocytic suspension either in the presence of a sample or without it. For this purpose the above-mentioned isotonic water solution containing standard erythrocytic suspension should be supplemented with glycine buffer at pH being 2-2.5 that induces acidic hemolysis. Detergent should be determined by the increase of acidic hemolysis rate in the presence of a sample. The method enables to improve detection in case of low concentrations of detergent in a sample.
EFFECT: higher sensitivity of detection.
2 ex, 2 tbl
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Authors
Dates
2005-03-10—Published
2003-07-28—Filed