METHOD FOR OBTAINING ARTIFICIAL SKIN MATRIX Russian patent published in 2005 - IPC

FIELD: medicine, experimental and clinical surgery, combustiology, transplantology, cosmetology.

SUBSTANCE: the present innovation deals with restoring artificial matrix of dermal-epidermal skin equivalent. The method, also, deals with mixing both collagen and chitosan by adding a surface-active substance and a binding agent followed by pouring the mixture obtained, its freezing followed by freeze drying and gamma-sterilization, moreover, before obtaining polyelectrolytic complex of collagen and chitosan one should successively supplement chitosan with aqueous solutions of 1.8 g/g dry chitosan of ascorbic acid, 5-100 mg/g dry chitosan of chondroitin sulfuric acid, 10-100 mg/g dry chitosan of hyaluronic (D-glucuronic) acid, 11-220 mcg/g dry chitosan of cattle serumal growth factor and 2.5-5 mg/g dry chitosan of heparin. The method provides to obtain a structure similar to skin that substitutes skin defect perfectly.

EFFECT: higher efficiency.

6 dwg,1 tbl