ACETYLCHOLINE DETERMINATION METHOD Russian patent published in 2005 - IPC

FIELD: analytical methods in biology.

SUBSTANCE: invention relates to chemical analysis methods for biological materials and, in particular, concerns a method for determining acetylcholine in biological specimens consisting in incubation of a specimen, centrifugation, take-off of supernatant, preparation of alkaline hydroxylamine reagent by adding equal volume of 3.5 n NaOH to hydroxylamine chloride solution, mixing of thus prepared reagent with test specimen, and addition of iron chloride solution followed by measurement of optical density. Invention is characterized by that, before addition of 3.5 n NaOH, hydroxylamine chloride solution is mixed with 10-14% solution of Na₂CO₃, incubation of specimen is performed after the latter is mixed with alkaline hydroxylamine reagent at 1:2 ratio for 90-120 min at 33-37°C, centrifugation is preceded by adding aqueous hydrochloric acid solution, and 0.74 M iron chloride solution is added to taken off supernatant.

EFFECT: increased determination accuracy.

2 tbl, 3 ex