SYNTHETIC ANTIGEN BASED ON COPOLYMERS OF N-VINYLPYRROLIDONE, CROTONIC ACID AND PARA-CROTONOYL AMINOPHENOL COMPRISING COVALENTLY JOINED 2-NAPHTHYLAMINE AS HAPTEN Russian patent published in 2005 - IPC

FIELD: organic chemistry, polymers, immunochemistry of high-molecular compounds.

SUBSTANCE: invention describes a synthetic hapten based on copolymers of N-vinylpyrrolidone, crotonic acid and p-crotonoyl aminophenol comprising covalently joined 2-naphthyl amine as a hapten of the following general formula:

wherein m = 88.5 mole%; n = 6.8-10.3 mole%, and l = 1.2-4.7 mole%. Invention provides specificity of antibodies raised against 2-naphthylamin as a hapten and possibility for preparing highly specific antibodies raised against carcinogen 2-naphthylamine as a hapten by immunization of rabbits with synthetic antigen comprising a copolymer of N-vinylpyrrolidone, crotonic acid and p-crotonoyl aminophenol as a macromolecular carrier. Also, invention provides possibility for practice using these antibodies for immunological recording 2-naphthylamine as a hapten in blood of industrial workers subjected for exposition with 2-naphthylamine. The proposed compound elicits antigenic features and can be used for detection of oncological risk among workers subjecting with contact with 2-naphthylamine.

EFFECT: valuable properties of antigen.

1 tbl, 1 dwg, 6 ex