FIELD: biotechnology, microbiology, genetic engineering, amino acids.
SUBSTANCE: invention describes a microorganism belonging to genus Escherichia used as a producer of L-amino acids with optimized level of expression of gene that effects in disposition of carbon flow, in particular, gene sucAB. Method involves stages for insertion of DNA fragments set into microorganism chromosome synthesized in vitro and comprising regulatory elements of gene expression instead the natural element of regulatory gene region to obtain population of microorganisms and selection of microorganisms showing the enhanced production of L-amino acids. Also, invention describes a method for preparing such L-amino acids as L-glutamic acid, L-proline, L-arginine, L-glutamine, L-leucine by using microorganisms with optimized level of expression of gene sucAB. Invention provides preparing L-amino acids with the high effectiveness degree.
EFFECT: improved preparing method.
15 cl, 1 dwg, 2 tbl, 5 ex
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