FIELD: biotechnology, microbiology.
SUBSTANCE: invention proposes the nutrient medium comprising enzymatic hydrolyzate of defatted cow milk proteins as a nutrient base with the content of amine nitrogen 120 mg% and concentration of hydrogen ions (pH value) 7.0 ± 0.2 in the following ratio of components, g/l: enzymatic hydrolyzate of defatted cow milk proteins, 0.3; potassium dihydrogen phosphate, 1.4; potassium hydrogen phosphate, 2.7; magnesium sulfate, 1.1; sodium chloride, 0.1, and distilled water, up to 1 l. Invention provides increasing yield of plague microorganism biomass of the vaccine antibiotic-resistant strain EB P2 in submerged culturing in liquid nutrient medium based on balanced composition of amino acids, peptides, carbohydrates and mineral salts.
EFFECT: improved and valuable properties of nutrient medium, increased yield of biomass.
2 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| LIQUID NUTRIENT MEDIUM FOR SUBMERGED CULTURING MICROORGANISM PLESIOMONAS SHIGELLOIDES, STRAIN 16 | 2002 |
|
RU2239654C2 |
| LIQUID NUTRITIONAL MEDIUM FOR CULTIVATION AND DISCHARGE OF BIOMASS OF VACCINE STRAIN OF PLAGUE MICROBE (YERSINIA PESTIS EV) | 2020 |
|
RU2745504C1 |
| NUTRIENT LIQUID MEDIUM FOR CULTURING PLAGUE MICROBE OF VACCINE STRAIN EB | 2010 |
|
RU2433170C1 |
| NUTRIENT BASE PRODUCTION METHOD AND NUTRIENT BASE FOR YERSINIA AND VIBRIO MICROORGANISMS FERMENTATION | 2007 |
|
RU2360962C2 |
| LIQUID NUTRIENT BROTH FOR DEEP CULTIVATION OF ICE-FORMING BACTERIA | 2006 |
|
RU2305706C1 |
| NUTRIENT MEDIUM FOR PLAGUE MICROBE ISOLATION | 0 |
|
SU1751211A1 |
| LIQUID NUTRIENT MEDIUM FOR CULTURING PLAGUE MICROORGANISM OF VACCINE STRAIN EB | 2004 |
|
RU2260620C1 |
| LIQUID NUTRIENT MEDIUM WITH INCREASED STORAGE PROPERTIES FOR OBTAINING BIOMASS YERSINIA PESTIS EV | 2022 |
|
RU2785826C1 |
| METHOD OF OBTAINING PREPARATION BASED ON VACCINE STRAIN OF PLAGUE MICROBE | 2012 |
|
RU2510825C2 |
| NUTRIENT MEDIUM FOR PESTILENTIAL MICROBE CULTIVATION | 2008 |
|
RU2380409C1 |
