FIELD: biotechnology, in particular transgenic plants and plant cells bearing chimeric gene in genome.
SUBSTANCE: invention relates to plant transformed with chimeric gene, which is expressed with simultaneous formation of cytotoxic protein. Chimeric gene includes promoter, which is induced in target region and/or adjacent thereto and is operably linked with sequence encoding ribosome inactivating maize protein or fragment thereof. Said encoding sequence includes recombinant maturated RIP, containing sequential domain-α and domain-β; recombinant RIP, containing as active fragment domain-α inactivating of maize protein ribosome alone; or recombinant RIP, containing as active fragment domain-β inactivating of maize protein ribosome alone; or encoding sequence presenting in SEQ.ID.No:1 or sequence being at least on 85 % homologous thereto. Also disclosed is plant cell transformed with chimeric gene, wherein chimeric gene includes promoter, which is induced in target region and/or adjacent thereto and is operably linked with sequence encoding ribosome inactivating maize protein or fragment thereof. Said encoding sequence includes recombinant maturated RIP, containing sequential domain-α and domain-β; recombinant RIP, containing as active fragment domain-α inactivating of maize protein ribosome alone; or recombinant RIP, containing as active fragment domain-β inactivating of maize protein ribosome alone; or encoding sequence presenting in SEQ.ID.No:1 or sequence being at least on 85 % homologous thereto.
EFFECT: plants with value characteristics, such as mail sterility, fruit asperity, flower doubling and eelworn resistance.
4 cl, 15 dwg, 3 tbl
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Authors
Dates
2006-04-27—Published
2001-10-15—Filed