METHOD OF PREPARING DIAGNOSTICUM TO REVEAL DIPHTHERIA TOXIN Russian patent published in 2006 - IPC G01N33/569 G01N33/543 

FIELD: medicinal microbiology.

SUBSTANCE: gist of invention resides in using polymer carrier prepared by a method of anionic polymerization of acryl aldehyde monomer in presence of sodium hydroxide as polymerization initiator, said polymer carrier being in the form of colored spherically-shaped equigranular particles 2±0.5 μm in diameter and having surface reactive aldehyde groups in quantity 1.3-1.6 μmol/g carrier, which carrier is washed with 0.1 M carbonate buffer solution having pH 9.0-9.2, centrifuged for 10 min at 3000 rpm, immobilized by suspending washed out carrier in 0.1 M carbonate buffer solution, pH 9.0-9.2, to which diphtheria antitoxic immunoglobuline is then added. Components are held in contact for 2 h at 20^°C, cooled to 7^°C, kept at this temperature for 16-18 h, and stabilized. Stabilization is effected by adding 1% gelatose solution in 0.9% sodium chloride solution, stirring for 2 h at 20^°C, and lyophilization of diagnosticum. Diphtheria toxin is revealed using space agglomeration reaction.

EFFECT: increased specificity of diagnosticum.

3 cl, 3 ex