FIELD: medicinal microbiology.
SUBSTANCE: method involves stage-by-stage immunization of rabbits with lipase from Pseudomonas sp. and preparing anti-lipase antibodies that are immobilized on polymeric microspheres. Then in the reaction of volume agglomeration in analyzed hemolytic strains of choleraic vibrios the property to produce lipase in interaction with anti-lipase antibodies immobilized on polymeric carrier is detected and the positive reaction is found confirming atoxigenic property of the analyzed strain being toxigenic - non-hemolytic strains don't show such ability but show the negative response reaction. Method provides reducing time for detection. Invention can be used in laboratory diagnosis of extreme danger infections.
EFFECT: improved method for detection.
4 cl, 1 tbl, 3 ex
