FIELD: biotechnology, veterinary, food processing industry.
SUBSTANCE: claimed method includes homogenization of crab hepatopancreas in 0.1 M ammonium acetate, containing 0.1-1 mM of calcium acetate at pH 6.4-7.9 followed by homogenate centrifugation. Ammonium sulfate is added to obtained supernatant up to 60-70 % saturation followed by centrifugation. Obtained enzyme complex in dissolved in distilled water in which 0.1-1 mM of calcium acetate is added to produce molecular solution followed by purification and concentration thereof. Enzymes are desalted on polyfiber membrane with pore size of 100 kDa. Supernatant containing collagenase inhibitor is washed with distilled water, heated up to 60-100°C and precipitate is separated by centrifugation. Enzyme solution is lyophilized.
EFFECT: new method for collagenase inhibitor production.
3 cl, 1 dwg, 1 tbl, 2 ex
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