ENZYME PREPARATION FROM HEPATOPANCREAS OF COMMERCIAL CRAB SPECIES AND METHOD FOR PRODUCTION OF THE SAME Russian patent published in 2006 - IPC C12N9/48 C12N9/64 

FIELD: multienzymatic preparation from sea animal raw materials, useful in biotechnology, medicine, cosmetology, veterinary, agriculture, etc.

SUBSTANCE: claimed preparation is obtained by homogenizing of hepatopancreas of commercial crab species in buffer solutions; solid contaminant removing by homogenate centrifugation and lipid flocculation with chitosan, wherein homogenate is additionally purified by centrifugation. Ultrafiltration of enzyme preparation solution is carried out on membrane permeable for substances of molecular mass 15 kDa or less and further on membrane non-permeable for substances of molecular mass 50 kDa or more. Solution having enzymatic activity is lyophilized. Multienzymatic preparation contains collagenolytic and metal-dependent proteinases having molecular mass less than 30 kDa, rybonucleases. Deoxyribonucleases, phosphodiesterases, phosphatases, amylases, lipases, and β-glucanases.

EFFECT: enhanced assortment of multienzymatic preparations.

2 cl, 4 ex