METHOD FOR DETERMINING AND SEPARATING FATTY ACIDS Russian patent published in 2007 - IPC G01N33/49 

FIELD: biological engineering.

SUBSTANCE: method involves treating blood with chloroform and methanol mixture taken in ratio of 2:1, carrying out lipid hydrolysis and methylation in sulfuric acid and methanol solution at 80°C within 3 h, cooling, processing methylated sample with hexane-containing solution, drying in nitrogen flow and carrying out fatty acids gas chromatography determination and separation on polar liquid diglycol succinate phase covering surface of 50 m long column of external diameter 0.32 mm, at chromatographic oven columns temperature equal to 140°C. Column is repeatedly washed out with chloroform, methanol, acetone and hexane before introducing diglycol succinate. Diglycol succinate is distributed over internal wall of capillary column using dynamic method so that diglycol succinate layer thickness makes 0.5 mcm. Then the column is purged with nitrogen during 5-6 h 3 days long at capillary column thermostat temperature equal to 200°C.

EFFECT: increased sensitivity in determining and separating fatty acids in biological fabrics.

2 dwg, 3 tbl