FIELD: analytical chemistry; capillary electrophoresis.
SUBSTANCE: method of electrophoresis separation of multiple component solutions is based upon washing capillary by electrolyte solution through, introducing sample into capillary, electrophoresis separating of components of sample in capillary under influence of voltage applied between ends of capillary, detecting and measuring times of output of separated components of sample. During process of washing capillary through by electrolyte solution, value of potential of flow is determined by measurement of potential difference between ends of capillary. Components of sample are identified according to measured value of flow potential and values of times of exit of sample components. Device has containers for electrolyte and for samples, capillary, aids for mounting capillary made for placement of ends of capillary into mentioned containers for electrolyte and samples, aids for generating flow of electrolyte through capillary, which aids are pneumatically connected with aids for mounting capillary, aids for generating voltage between ends of capillary, detector connected with capillary, signal control and processing system and aids for measuring flow potential. The aids and units mentioned above are made in such a way that they are able of closing the measuring electric circuit, which consists of electrolyte in containers and in capillary, as well as open it during electrophoresis separation when capillary is washed through by electrolyte.
EFFECT: improved reproducibility; higher productivity.
10 cl, 6 dwg
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Authors
Dates
2007-05-27—Published
2006-02-26—Filed