FIELD: medicine, immunology, clinical laboratory diagnosis.
SUBSTANCE: method involves using the usual solid-phase enzyme immunoassay based on chemical binding antigen with microplate surface followed by placing antibodies-containing biological fluid on microplate, incubation, carrying out the color reaction and spectrophotometric evaluation of the color reaction indices. The end antigen is prepared by enzymatic hydrolysis of human normal tissue samples containing stromal cells of endometrial tissue. The enzymatic hydrolysis reaction is terminated by addition of chelating agent. Prepared mixed suspension of cells is centrifuged in continuous density gradient resulting isolation of stromal cells. Microsomal fraction of stromal cells is used as the end antigen that is diluted and bound covalently with microplate well surface. Then calibrating material prepared on basis of standard preparation antibodies specific to the end antigen is added into wells set, and biological fluid samples for analysis are added into another wells set. After carrying out the usual solid-phase enzyme immunoassay and using spectophotometry data a calibrating curve is plotted and the concentration of specific antibodies is determined using this curve. Invention provides the development of method for assay of antibodies amount specific to endometrial tissue stromal cells exceptionally that gives possibility for clinical diagnosis of autoimmune syndrome. Invention can be used in gynecology and obstetrics for diagnosis of autoimmune syndrome accompanying to clinical picture of endometriosis and other diseases in female reproductive system.
EFFECT: improved method of quantitative assay.
2 cl, 2 dwg, 2 ex
