METHOD FOR MANUFACTURING VACCINE AGAINST PSEUDOMONOSIS IN SWINE Russian patent published in 2007 - IPC A61K39/104 

FIELD: veterinary medicine.

SUBSTANCE: the innovation deals with cultivation of antigen, inactivation with formalin and introduction of aluminum hydroxide. As antigen one should apply epizootic strains of Pseudomonas aeruginosa of serogroups O1, O3, O4, O6, O11, O19 that provide "S"-forms of colonies, then one should prepare suspension, fulfill inoculation onto differentially-diagnostic media, isolate pure cultures of Pseudomonas aeruginosa agents of O1, O3, O4, O6, O19 serogroups that provide "S"-shaped forms of colonies. Then they should be grown separately for about 24-36 h upon Hottinger's broth at pH being 7.2-7.4 and dissolved at 36-37°C with physiological solution up to concentration of 10 bln microbial cells/ml. Then comes inactivation with formalin that contains 36% formaldehyde, not less to achieve final concentration ranged 0.3-0.4% to be kept for 14 d at about 37-37.5°C. Moreover, the culture should be mixed at equal volumes and at uninterrupted mixing one should introduce 3%-aluminum hydroxide solution at not more than 25% against the volume of cultures mixture to be thoroughly mixed, packed and sealed. The innovation enables to obtain highly immunogenic vaccine that forms tense immunity of 9 mo duration, not less and causes no post-vaccinal complications.

EFFECT: higher efficiency of manufacturing.

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