METHOD FOR MANUFACTURING OF POLYVALENT SERUM AGAINST PSEVDOMENOSIS OF AGRICULTURAL ANIMALS Russian patent published in 2009 - IPC A61K39/40 

FIELD: veterinary science.

SUBSTANCE: method includes hyperimmunisation of stud bulls with polyvalent antigen, which is produced by cultivation of epizootic strains grown separately in Hottinger broth, having pH 7.2 -7.4, and mixed in equal proportions with further inactivation with formalin, blood draw to prepare serum, separation of serum with further preservation and control for activity. At the same time as epizootic strains they use Pseudomonas aeruginosa of serotypes 01, 02, 03, 04, 06, 010, 011, 013, 018, 019, and immunisation is carried out in cycles with growing doses of antigen. Antigen is injected subcutaneously not more than 14 times in 3-4 days for 51 days, starting from 5 ml, besides two subsequent doses each time are increased twice, and the remaining ones - 1.3 times more compared to each previous one, and the last four doses of not more than 150 ml. Blood draw in servicing animals for control definition of titres is done in 8, 22, 32 days from the beginning of antigen injection. In order to produce serum, blood is drawn at 52-53 day from the start of antigen injection.

EFFECT: serum has high efficiency for treatment of pigs and calves, as well as for sows.

2 cl, 1 tbl, 1 ex