POLYVALENT VACCINE AGAINST PSEUDOMONASIS IN FARM ANIMALS Russian patent published in 2007 - IPC A61K39/104 A61P31/04 

FIELD: veterinary medicine.

SUBSTANCE: the vaccine suggested contains cell suspension of pure cultures of Pseudomonas aeruginosa agent as antigens that belong to ten sero-groups N18(01); N47(02); N37(03); N19(04); N2(06); N13(010), N70(011); N66(013); N29(018); N28(019) obtained due to selecting parenchymal affected organs from dead animals out of local epizootic focus, inoculating onto differential-diagnostic media and isolating pure cultures of agents. Raising of pure cultures of agents should be carried out separately in meat-peptone broth and agar up to concentration of every sero-group of microbial cells to be about 9.5-10.5 bln/ml, then one should mix them in equal ratios. As a conservant one should introduce formalin, as an adjuvant - aluminum hydroxide at the following ratio of components, weight%: cell suspension of pure culture of Pseudomonas aeruginosa agent of ten sero-groups N18(01); N47(02); N37(03); N19(04); N2(06); N13(010), N70(011); N66(013); N29(018); N28(019) isolated out of local epizootic focus in nutritive medium at the titer being about 9.5-10.5 bln microbial cells/ml 84.6-89.5; formalin 0.4-0.5; aluminum hydroxide - the rest. The suggested vaccine is of high immunogenic activity.

EFFECT: higher efficiency.

5 ex, 1 tbl