FIELD: biotechnology, microbiology, biochemistry.
SUBSTANCE: invention relates to a novel strain of microorganisms producing cyclodextrin glucanotransferase (CGTase; EC 2.4.1.19) with the enhanced specificity with respect to formation of beta-cyclodextrin. The strain of bacterium Paenibacillus campinasensis IB-10155 is deposited and stored in Collection of microorganisms in Biology Institute UNTS RAN. Activity of CGTase in cultural fluid is estimated to be 5.8 U/ml wherein 1 unit of activity is determined as amount of CGTase catalyzing formation of 1 mcM of ACB beta-cyclodextrin per 1 minute from 2% starch solution at pH = 6.0. Part of beta-cyclodextrin in sum of other cyclic products exceeds 70%. Invention provides high stability of CGTase catalyzing the transformation reaction of starch to beta-cyclodextrin at relative constant part of this product to sum of other cyclic dextrins in early steps of reaction.
EFFECT: valuable properties of strain.
2 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| BACTERIUM STRAIN PAENIBACILLUS MACERANS AS PRODUCER OF ALPHA-CYCLODEXTRIN GLUCANOTRANSFERASE | 2006 |
|
RU2303062C1 |
| STRAIN OF BACTERIA BACILLUS SP - A PRODUCER OF @@@-CYCLODEXTRINTRANSFERASE | 0 |
|
SU1738852A1 |
| STRAIN OF BACTERIUM BACILLUS SP FOR PREPARING AGENT USED AGAINST FUNGOUS PATHOGENS OF CEREALS DISEASES | 1989 |
|
RU1743019C |
| METHOD FOR ISOLATION AND SELECTION OF MICROORGANISMS AS PRODUCERS OF CYCLODEXTRIN GLUCANOTRASNFERASE, STRAIN OF MICROORGANISM BACILLUS CIRCULANS B-65 NCAIM (P) 001277 (B-65) AS PRODUCER OF EXTRACELLULAR CYCLODEXTRIN TRANSFERASE, CYCLODEXTRIN GLUCANOTRANSFERASE OBTAINED FROM ITS AND ITS APPLYING FOR PREPARING CYCLODEXTRIN | 2000 |
|
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| STRAIN OF BACTERIUM BACILLUS LICHENIFORMIS AS PRODUCER OF THERMOSTABLE AMYLOLYTIC AND PROTEOLYTIC ENZYMES | 1998 |
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| STRAIN OF BACTERIA PAENIBACILLUS SPECIES - A PRODUCER OF XYLANASE | 2017 |
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| PRODUCER OF COMPLEX OF CHITINOLYTIC ENZYMES AND LAMINARINASE | 2001 |
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RU2213773C2 |
