FIELD: biotechnology, microbiology, biochemistry. SUBSTANCE: invention relates to the strain-antagonist Bacillus sp. 739 exhibiting a novel property ability to form complex of extracellular chitinolytic enzymes including chitinase, chitosanase and laminaranase. The strain is deposited in collection of microorganisms of NII of agricultural microbiology at number 132. Morphological features of culture have been studied using media with beef-extract and potato agar. In the first case the strain forms mucous, convex, unclear colonies converting then to the folded form with height rim by the edge. Small mucous, convex and unclear colonies are formed on potato agar. On the third day colonies transform to folded ones. The strain is aerobic, grow good in the range of temperature from 30 C to 45 C, assimilates glucose, sucrose, arabinose, mannitol, glycerol and ethanol, hydrolyses starch but doesn't form gases in fermentation of sugars and forms cyclodextrine crystals on starchy media. The strain gives positive Foges-Proscauer reaction (formation of acetylmethylcarbinol) but doesn't form indole and is able to grow in the pH value ranges 5.0-8.5. By physiological and biochemical features the strain doesn't correspond to neither strains described in Bergey's Manual of Systematic Bacteriology and the strain is identified as Bacillus sp. 739. The total chitinase activity in cultural fluid is 0.15-0.20 U/ml and activity of chitosanase and laminarinase is measured to be about 0.10 and 2.0-2.5 U/ml of cultural fluid, respectively. This enzyme preparation is able to lyse cellular walls of some mycelial fungi actively. EFFECT: valuable properties of complex. 2 dwg, 1 tbl, 3 ex
