FIELD: biotechnology, genetic engineering.
SUBSTANCE: invention relates to a plasmid vector able to autonomic replication in the strain Escherichia coli K-12 at any temperature. Plasmid comprises region or with mutation, gene rep from pMW119 and DNA fragment. DNA fragment can be integrated into chromosome of microorganism belonging to Escherichia coli W or Escherichia coli B as result of homologous recombination. Plasmid is fused into a microorganism belonging to Escherichia coli W or Escherichia coli B followed by preparing amino acid. Also, invention relates to strains Escherichia coli DH5α/pMTS11910 (FERM BP-6904) and Escherichia coli DH5α/pMTS11914 BP-6905) designated for storage the plasmid vector. Strains Escherichia coli WLA-131 (FERM BP-6902) and Escherichia coli WL-1133 (FERM BP-6903) are producers of amino acid leucine. Invention provides carrying out superexpression of gene and to obtain stable plasmids.
EFFECT: valuable biological properties of plasmids and strains.
13 cl, 2 dwg, 1 tbl, 5 ex
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