METHOD FOR BACTERIOLOGICAL DETECTION OF NOCARDIOMORPHOUS ACTINOMYCES Russian patent published in 2008 - IPC C12Q1/04 

FIELD: biotechnology, veterinary microbiology.

SUBSTANCE: method involves preparing pair samples from the pathological material sample. The first sample is poured with 0.5%, and the second sample - with 2.0% aqueous solution of the preparation "Leseptik" taken in the volume ratio 1:2 followed by their exposition for 3 h and 15 min, respectively, at room temperature. Then the "Leseptik" preparation solution is poured off, washed out twice with isotonic sodium chloride solution, and suspension is prepared that is inoculated on solid egg-containing media and grown at temperature +37°C. Invention provides accelerated primary indication and differentiation of positive reactions for PPD-tuberculin for mammals caused by mycobacterium tuberculosis from positive reactions for PPD-tuberculin for mammals caused by corynebacteria, rhodococci and nontuberculosis mycobacteria.

EFFECT: improved method of detection.

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