METHOD FOR SCREENING OF PROBIOTIC PREPARATIONS FOR NOCARDIOFORM ACTINOMYCETES Russian patent published in 2012 - IPC C12Q1/02 C12N1/20 

FIELD: medicine.

SUBSTANCE: mice are infected by the oral introduction of a suspension 0.1 ml of a two-day swine Rhodococcus equi, pathogenic of culture containing 5×10⁷ CFU in normal saline 1.0 ml. The infection is followed the oral five-fold introduction of a probiotic preparation 1-5×10^5-6 CFU in 1.0 ml every 24 hours; in 5 days after the infection, the mice are killed, and dense egg medium are inoculated of the internals suspensions. In 1-2 cultivation days at optimal temperature, but not later than in 5 days, the grown colonies are controlled by acid fast stain. Antagonist activity is evaluated by a growth block index that is a relation of Rhodococcus colony count grown at inoculation of the internals suspensions of the animals prescribed with no probiotic preparations to Rhodococcus colony count grown at inoculation of the internals suspensions of the animals prescribed with the probiotic preparations. The efficacy of the probiotic preparation is concluded if the value exceeds 3.

EFFECT: invention provides fast and nocardioform actinomycete adequate screening of the probiotic preparations in vivo.

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