FIELD: gene engineering.
SUBSTANCE: invention relates to plant gene engineering. Artificial promoter is a chimeric molecule of recombinant DNA and produces high expression level of DNA molecule combined with 3'-end when injected into plant cells. Basic genetic elements of chimeric molecules include promoter nucleus with consensus TATA-box and exon/intron/exon area and translation enhancer. All of these elements are structured artificially. Regulatory elements of transcription may be built into the given promoter in reverse direction for organo-specific, tissue-specific or specific stage of expression development. In order to increase levels of built artificial genetic elements translation/transcription, they may be functionally inserted between any promoter, which is active in cell or any DNA sequence.
EFFECT: increase of translation/transcription levels.
68 cl, 12 dwg, 7 tbl, 4 ex
