FIELD: gene engineering, medical and biological industry, therapeutic proteins, gene therapy.
SUBSTANCE: invention relates to variants of DNA construct to integrate chromosomal DNA of cell, residing before endogenic gene of interest, into certain site (i.e. target site). Said integration is carried out by homological recombination between sequence of abovementioned target site and "guiding" sequence incorporated in structure of said construct. DNA construct includes also exogenic promoter and exon with unpaired donor splicing site or exon with donor site and intron with acceptor splicing site. Embedding of such construct unto cell genome makes it possible to produce new (non-natural) transcription units and to directionally change of any endogenic gene expression.
EFFECT: new agents for gene therapy.
60 cl, 15 dwg, 7 tbl, 9 ex
