FIELD: biotechnologies.
SUBSTANCE: generic source of eubacteria in a clinical sample is detected and identified using real-time PCR assay. For that purpose, the eubacteria rRNA gene 16S segment containing a conservative region and a divergent region is amplified in the presence of at least two fluorogenic probes containing reporter dyes and quencher dyes. One of the fluorogenic probes is selected so that it is hybridised with the said conservative region. The other fluorogenic probe is selected so that it is hybridised with the divergent region of the generic eubacteria gene, presumably present in the clinical sample. In one of the assay options, the PCR mixture is filtered before adding a sample containing the DNA matrix. For the amplification of the rRNA gene 16S segment, a pair of primers is created, complementary to the regions of the said gene, which are conservative for various eubacteria types.
EFFECT: this primer pair and said fluorogenic probes are used in set for detection and identification of generic source of bacteriemia in clinical source.
52 cl, 5 ex, 5 tbl, 5 dwg
