FIELD: biotechnology.
SUBSTANCE: invention relates to a method for detecting DNA of Mollicutes microorganisms by RT-PCR in human and animal biological material. In said method, as a method for determining the presence of nucleic acids of microorganisms of the Mollicutes class two synthetic oligonucleotide primers and a fluorescent-labeled probe specific to 16S rRNA gene region are used in the analyzed sample during PCR.
EFFECT: present invention provides fast reliable high-sensitivity detection of Mollicutes microorganisms by RT-PCR in human and animal biological material.
1 cl, 1 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND A FLUORESCENTLY LABELED PROBE FOR THE DNA FRAGMENT OF THE 16S RIBOSOMAL RNA GENE OF UREAPLASMA GENUS OF MYCOPLASMATACEAE FAMILY OF MYCOPLASMATALES ORDER (UREAPLASMA SPP.) OF MOLLICUTES CLASS FOR THE DETECTION OF PATHOGENIC UREAPLASMAS OF CATS AND DOGS | 2022 |
|
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| METHOD OF IDENTIFYING DNA OF UREAPLASMA DIVERSUM BACTERIA IN BOVINE SEMEN BY REAL-TIME PCR | 2021 |
|
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| METHOD FOR MEDIATED DETERMINATION OF TITRE OF INFECTIOUS ACTIVITY OF MYCOPLASMA GALLISEPTICUM BY PCR IN REAL TIME MODE | 2023 |
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| METHOD FOR DETECTING THE PRESENCE OF MUTATIONS LEADING TO MYCOPLASMA GENITALIUM RESISTANCE TO MACROLIDE AND FLUOROQUINOLONE ANTIBIOTICS | 2019 |
|
RU2725477C1 |
| SYSTEM OF OLIGONUCLEOTIDE PRIMERS AND PROBE FOR DETECTING DNA MYCOPLASMA BOVIS | 2019 |
|
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| SYSTEM OF OLIGONUCLEOTIDE PRIMERS AND PROBE FOR DETECTING DNA MYCOPLASMA BOVIGENITALIUM | 2019 |
|
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| KIT FOR DETECTING LEPTOSPIROSIS IN BIOLOGICAL MATERIAL THROUGH REAL-TIME POLYMERASE CHAIN REACTION (RT-PCR) METHOD | 2019 |
|
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| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND A METHOD OF HIGHLY SENSITIVE DETECTION OF AFRICAN SWINE FEVER VIRUS DNA BY LOOP ISOTHERMAL AMPLIFICATION IN THE PRESENCE OF INTERNAL CONTROL SAMPLE DNA | 2022 |
|
RU2799410C1 |
| METHOD FOR TARGET AMPLIFICATION OF HUMAN REPRODUCTIVE ORGANS INFECTORS GENOMES FOR SIMULTANEOUS IDENTIFICATION OF INFECTORS WITH PRIMER SET | 2015 |
|
RU2625006C1 |
| KIT OF REAGENTS FOR DETECTION OF NEISSERIA GONORRHOEAE DNA AND USE THEREOF | 2015 |
|
RU2595398C1 |
