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2 333 956

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(51)

IPC

C12N1/21(2006-01-01)

(21) (22)

Application

2006140913/13, 2006-11-21

(24)

Start date

2006-11-21

(22)

Actual filing date

2006-11-21

(45)

Published

2008-09-20

(72)

Inventor

Severin Sergej Evgen'EvichSeverin Evgenij SergeevichGorokhovets Neonila Vasil'EvnaSavvateeva Ljudmila Vladimirovna

(73)

Holder

Avtonomnaja Nekommercheskaja Organizatsija Molekuljarnoj Diagnostiki"

STRAIN E coli BL21(DE3)/pET-hHSP70 - PRODUCENT OF PROTEIN OF HEAT SHOCK IN HUMANS HSP70 Russian patent published in 2008 - IPC C12N1/21

Abstract RU 2333956 C1

FIELD: chemistry.

SUBSTANCE: invention relates to the field of biotechnology and can be used in medico-biological industry. On the basis of vector pET28a(+) plasmid DNA pET-hHSP70, which contains nucleotide sequence encoding protein of heat shock in humans hHSP70A1B is constructed. By transforming cells of E.coli by plasmid DNA pET-hHSP70 strain of E.coli BL21(DE3)/pET-hHSP70 - producent of recombinant form hHSP70 - is obtained.

EFFECT: obtaining recombinant protein hHSP70 with a high output (in amount of 30-40% of total content of cell protein) and simplifying its isolation and purification.

3 dwg, 3 ex

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RU 2 333 956 C1

Authors

Severin Sergej Evgen'Evich

Severin Evgenij Sergeevich

Gorokhovets Neonila Vasil'Evna

Savvateeva Ljudmila Vladimirovna

Dates

2008-09-20—Published

2006-11-21—Filed