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IPC

C12N15/00(2006-01-01)

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Application

2013109368/10, 2013-03-04

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Start date

2013-03-04

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Actual filing date

2013-03-04

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Published

2014-08-27

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Inventor

Petrovskaja Lada Evgen'EvnaShingarova Ljudmila NikolaevnaGapizov Sultan ShakhbanovichKrjukova Elena AleksandrovnaBoldyreva Elena FilippovnaJakimov Sergej AleksandrovichDolgikh Dmitrij AleksandrovichKirpichnikov Mikhail Petrovich

(73)

Holder

Federal'Noe Gosudarstvennoe Bjudzhetnoe Uchrezhdenie Nauki Institut Bioorganicheskoj Khimii Im. Akademikov M.M. Shemjakina I Ju.A. Ovchinnikova Rossijskoj Akademii Nauk Ran)

RECOMBINANT PLASMID DNA ENCODING HYBRID POLYPEPTIDES WITH PROPERTIES OF RED FLUORESCENT PROTEIN mCherry TO PRODUCE HYBRID FLUORESCENT PROTEINS IN Escherichia coli Russian patent published in 2014 - IPC C12N15/00

Abstract RU 2527171 C1

FIELD: biotechnology.

SUBSTANCE: invention is recombinant plasmid DNA encoding hybrid polypeptides with the properties of red fluorescent protein mCherry. The recombinant plasmid DNA pChFN3 encodes the hybrid protein ChFN3 which has the properties of red fluorescent protein mCherry and 10 domain of human fibronectin of type III, and the recombinant plasmid DNA pChTNF encodes the hybrid protein ChTNF which has the properties of red fluorescent protein mCherry and TNF. The present invention provides efficient production of hybrid proteins pChFN3 and ChTNF with the properties of red fluorescent protein mCherry in the strain E.coli BL21 (DE3).

EFFECT: invention enables to obtain the target protein in high yield by adding a smaller amount of inducer.

2 cl, 3 dwg, 8 ex

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RU 2 527 171 C1

Authors

Petrovskaja Lada Evgen'Evna

Shingarova Ljudmila Nikolaevna

Gapizov Sultan Shakhbanovich

Krjukova Elena Aleksandrovna

Boldyreva Elena Filippovna

Jakimov Sergej Aleksandrovich

Dolgikh Dmitrij Aleksandrovich

Kirpichnikov Mikhail Petrovich

Dates

2014-08-27—Published

2013-03-04—Filed