FIELD: medicine.
SUBSTANCE: in this method process native plasma or blood serum chemical reagents. Consistently bring in Phenolum to its final concentration (0.7±0.2)%, condition within 5 days at temperature (10±2)°C and then add ammonia sulphate to concentration of 50 % from saturation. Store the obtained protein suspension at temperature (10±2)°C and selectively transfer in immunoglobulin and albuminous half-finished products by centrifugation of sulphatic suspensions, for the purpose of separate obtaining of immunoglobulin deposits and albumins, extract the deposits in 1-2% solution of calcium chloride, remove slightly solublecalcium sulfate using centrifugation and reprecipitate target proteins from the supernatant solution with polyethyleneglycol-6000.
EFFECT: invention allows improving conditions of storage of plasma or blood serum without a denaturation of immunoglobulins and albumins on a basis of resource-saving methods without use of the expensive equipment and accessories for excision of impurity of technological reagents.
2 cl, 1 dwg, 2 tbl
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Authors
Dates
2008-11-20—Published
2006-09-04—Filed