METHOD OF SUNFLOWER MILDEW AGENT PRESERVATION Russian patent published in 2009 - IPC A01H4/00 

FIELD: agriculture.

SUBSTANCE: tissues of the mildewed plants are collected to obtain mildew agent. Then seeds of mildew-sensitive sunflower grade are artificially infected with the mildew agent. The infected preservator of the infection is kept under low temperature and is used as a source of primary infection. The collected tissues of the mildewed plants are placed in a damp chamber for 15-18 hours, taken out to make water suspension of the mildew agent by water washing away of white deposits from the collected tissues into a vessel, the deposits contain mildew fungi mycelium and oospores. The obtained suspension is concentrated up to 90000-100000 zoosporangiums per 1 ml of water. Seeds of mildew-sensitive sunflower grade are placed in the suspension, held for 24 hours under the temperature of 16-18°C, dried under the room temperature up to air-dry state and put into sterile flasks with a plug for storage, further they are used as a primary mildew infection source, i.e. for inoculum production.

EFFECT: possibility to preserve sunflower mildew agent for 2 years and use it as primary mildew infection source at any time of the year.

11 cl, 1 tbl, 1 ex