FIELD: medicine.
SUBSTANCE: method of obtaining laccase enzyme medicine involves sustaining cultivation parametres depending on dissolved oxygen concentration in medium. Temperature of 25-27°C and pH within 3.0-7.0 are maintained from the start of cultivation until dissolved oxygen concentration falls to 50%. After further decrease of dissolved oxygen concentration to 5% temperature is increased to 27-29°C and uncompensated pH range is narrowed to 4.0-5.0. At reduction of dissolved oxygen concentration to minimum level and its further increase, temperature of 30-33°C and pH of 4.0-6.0 are maintained.
EFFECT: enhanced activity of culture fluid containing laccase.
1 tbl, 3 ex
| Title | Year | Author | Number |
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SU1068477A1 |
