METHOD OF PRODUCING OF LACCASE Russian patent published in 2009 - IPC C12N9/02 C12N9/58 

FIELD: chemistry, biochemistry.

SUBSTANCE: proposed method includes deep cultivation of the strain of fungi Trametes hirsuta (Wulfen) Pilat, OOO PKF "BIGOR" CF-28 on a nutrient medium, separation of the fungi biomass, membrane concentration of the filtrate. The nutrient medium composition incorporates growth factors in concentration of 0.1-1.02 wt % and wheat flour in concentration of 2.0-4.0 wt % is used as a source of carbon. The cultivation is carried out at 28 to 34°C, pH 4.0 to 4.5, the concentration of dissolved oxygen making at least 1.5 to 2.0 mg·dm^-3. The membrane concentration is effected with the membrane element pore sizes varying from 0.1 to 0.5 microns, at a pressure difference of 1.0 to 4.0 MPa and temperature of 20 to 40°C. Laccase activity makes some 140 to 200 ME.

EFFECT: production of fermented laccase preparation with high activity.

2 ex