METHOD OF PREPARATION OF LIQUID OR DRY CONCENTRATE OR BIFIDOBACTERIA FERMENT Russian patent published in 2008 - IPC C12N1/20 A61K35/74 A23C9/12 

Abstract RU 2326940 C2

FIELD: technological processes; medicine.

SUBSTANCE: method of preparation of liquid or dry concentrate or Bifidobacteria ferment includes introduction of inoculation dose of Bifidobacteria culture Bifidobacterium bifidum 8-3 ("ВКПМ Ас- 1248"), Bifidobacterium longum "ЯЗ" ("ВКПМ Ас-1252"), Bifidobacterium adolescentis Bl ("ВКПМ Ас-1243") into nutrient medium. The medium contains, (mass %): lactopeptone 5.0-7.0; muriatic cysteine 0.001-0.005; autolysate or extract of bakery yeast 2.0-4.0; non-fat milk with content of non-fat milk solids of not more than 8.0% - the rest up to 100%. Biomass of bifidobacteria is grown at (37.0-38.0)±0.5°C until concentrate of bifidobacteria is obtained with at least 1011 CFU/ml. The product is cooled and packaged, or protective medium is introduced into it, and it is dried liophilically in sterile conditions.

EFFECT: provides for the possibility of preparation of stable uniform biomass of bifidobacteria for production of preparations with high bifidobacteria titre.

5 cl, 5 tbl, 4 ex

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RU 2 326 940 C2

Authors

Bajbakov Vladimir Ivanovich

Molokeev Aleksej Vladimirovich

Karikh Tat'Jana Leonidovna

Jatsentjuk Raisa Mikhajlovna

Nikulin Leonid Georgievich

Kuslij Aleksandr Georgievich

Mironenko Vjacheslav Vladimirovich

Jasudis Galina Vladimirovna

Guseva Irina Aleksandrovna

Dates

2008-06-20Published

2006-04-25Filed