FIELD: technological processes; medicine.
SUBSTANCE: method of preparation of liquid or dry concentrate or Bifidobacteria ferment includes introduction of inoculation dose of Bifidobacteria culture Bifidobacterium bifidum 8-3 ("ВКПМ Ас- 1248"), Bifidobacterium longum "ЯЗ" ("ВКПМ Ас-1252"), Bifidobacterium adolescentis Bl ("ВКПМ Ас-1243") into nutrient medium. The medium contains, (mass %): lactopeptone 5.0-7.0; muriatic cysteine 0.001-0.005; autolysate or extract of bakery yeast 2.0-4.0; non-fat milk with content of non-fat milk solids of not more than 8.0% - the rest up to 100%. Biomass of bifidobacteria is grown at (37.0-38.0)±0.5°C until concentrate of bifidobacteria is obtained with at least 1011 CFU/ml. The product is cooled and packaged, or protective medium is introduced into it, and it is dried liophilically in sterile conditions.
EFFECT: provides for the possibility of preparation of stable uniform biomass of bifidobacteria for production of preparations with high bifidobacteria titre.
5 cl, 5 tbl, 4 ex
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Authors
Dates
2008-06-20—Published
2006-04-25—Filed