FIELD: biotechnologies, veterinary.
SUBSTANCE: method includes determining polymorphism of gene Mx1. Isolation of DNA, with its further amplification by method of polymerase chain reaction (PCR) using two primers, is carried out. Further amplificate restriction with restrictase Rsal and detection of restriction product is carried out. Conclusion about genetic predisposition of hens to viral infections is made by appearance in electrophoresis gel of amplified DNA fragments with diameter 76 of nucleons pairts.
EFFECT: possibility of highly accurate, simple and cheap analysis of hen predisposition to viral infection.
2 cl, 1 dwg, 2 tbl, 1 ex
